Table 1 RT-PCR primer sequences and annealing temperatures for validation of microarray expression data.
Gene symbol | PCR primer sequences | Annealing (°C) | PCR-based expression | Chip-based expression |
|---|---|---|---|---|
CTNNB1 | 1 st CCTGGTGCTGACTATCCAGT | 52 | Down | Down |
2nd ACTTCCACACATGATCACAT | ||||
VEGF | 1 st GAGGGCAGAATCATCACGAA | 62 | Down | NA |
2nd AACGCTCCAGGACTTATACC | ||||
Cox2 | 1 st CGAGGTGTATGTATGAGTGT | 60 | Down | NA |
2nd AGGGAGTCGGGCAATC | ||||
Id2 | 1 st CCGCTCGAGACCATGAAAGCCTTCAGTCCCGTGAGG | 61 | Down | NA |
2nd CGGGATCCTTGCCACACAGTGCTTTGCTG | ||||
Gastrin | 1 st CGGAATTCACCATGCAGCGACTATGTG | 58 | Down | NA |
2nd CGGGATCCATGTTCTCATCCTCAGCACT | ||||
MMP7 | 1 st TGTTAAACTCCCGCGTCATA | 58 | Down | NA |
2nd GCGTTCATCCTCATCGAAGT | ||||
CD44 | 1 st CCATCCTCGTCCCGTCCT | 56 | Down | NA |
2nd GCCCTTCTATGAACCCATACCT | ||||
MYBL2 | 1 st CTCATCTTGTCCTGAGGTGT | 56 | Up | Up |
2nd TGAGGCCAGGCCTGACGTG | ||||
BAG3 | 1 st AGCCATCCTGGAGAAGGTAC | 56 | Up | Up |
2nd GGCACCCATCTCCATGATTG | ||||
PTEN | 1 st CCACCACAGCTAGAACTTA | 52 | Up | Up |
2nd CAACAGTGCCACTGGTCTA | ||||
PDCD6IP | 1 st CTCGGCTCTGTTAGTGTAAC | 52 | Up | Up |
2nd CACTTCTGACTAGTTCCTCT | ||||
HIF1A | 1 st GATACCAACAGTAACCAACCT | 52 | Up | Up |
2nd CCGGTTTAAGGACACATTCT | ||||
BAG2 | 1 st GAAGATCTATGGCTCAGGCGAAGAT | 56 | Up | Up |
2nd ATTTGCGGCCGCCTAATTGAATCTGCTTTCAGC | ||||
DAP3 | 1 st CCTCCTAGTGGCCGTGGATG | 56 | Up | Up |
2nd GCGTTACTTAGGAACAGCAG | ||||
β-actin | 1 st GGCGGCACCACCATGTACCCT | 56 | Control | NA |
2nd AGGGGCCGGACTCGTCATACT |