Figure 5

HOMUR cells that are resistant to DCA-induced apoptosis exhibit anchorage independent growth. HCT116, Homur7, Homur11, and Homur17 cells were plated into six well trays in 0.6% LMP agarose as described in Methods and the cells allowed to grow for 2.5 weeks. Colonies were then stained and counted. Bars depict the average number of clones per well from four independent experiments done in triplicate +/- standard deviation. Asterisks indicate a significant difference (p < 0.05) when compared with parental HCT116 cells.