Figure 1

The majority of HOMUR cell lines exhibit resistance to DCA-induced and hyoDCA-induced apoptosis. (A) HOMUR cells were incubated with 500 micromolar DCA for 24 hours and the fraction of apoptotic cells determined as described in Methods. The apoptotic value for each of the 41 HOMUR lines is depicted as a dot along a scale extending from 0 to 60% apoptosis. The extent of apoptosis obtained for parental HCT116 cells is indicated with an arrow at the right. Also on the right are dots representing individual HOMUR cell lines with apoptotic values greater than 60%. (B) HOMUR cells were incubated with 500 micromolar hyoDCA for 48 hours and the fraction of apoptotic cells determined as in panel A. Each dot represents a single HOMUR cell line and its placement is representative of the apoptotic value for that cell line. The apoptosis obtained using HCT116 cells is indicated with an arrow at the right. In addition, HOMUR lines with apoptotic values greater than 40% are represented as dots to the right of the graph.