GAG-hed down-regulates the HPV18 LCR activity in transgenic mice. Female transgenic mice were used to test GAG-hed effects on the expression of lacZ reporter gene whose transcription is driven by HPV18-LCR. GAG-hed vaginal washes (10 mg/ml, 50μl) were applied twice a day for 6 days. Six vaginal and tongue specimens were stained or tested for β-galactosidase activity. (A) Relative activity is expressed as the relationship between β-galactosidase units in vaginal (V) and tongue (T) tissues (3 mice) for the unique concentration employed compared with the effects of saline application (3 mice). (B) Images obtained from vaginal samples stained for β-galactosidase using X-Gal. Left panel, vaginal sample treated with saline solution (control); right panel, vagina treated with GAG-hed.