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Table 1 Expression of Bcl-2, Bcl-XL and mutant proteins in MCF-7 cells.

From: Bcl-XL is qualitatively different from and ten times more effective than Bcl-2 when expressed in a breast cancer cell line

Cell Clone

Number of Independent Experiments

Expression of Bcl-2 or Bcl-XL proteins (ng/μg total protein) no drug

Expression of Bcl-2 or Bcl-XL proteins (ng/μg total protein) at the EC50 dose of doxorubicin

EC 50 doxorubicin (μM)

Inhibition of PARP degradation.

Neo

5

n.d.

n.d.

1.9 ± 0

Bcl-2 (#2)

6

4.5 ± 1.0

2.5 ± 0.1

22.8 ± 3.3

Bcl-2 (#5)

7

3.6 ± 0.7

1.6 ± 0.1

30.6 ± 1.0

Bcl2-acta (#24)

7

1.2 ± 0.3

0.2 ± 0.02

13.2 ± 1.7

Bcl2-cb5 (#18)

5

1.5 ± 0.4

1.3 ± 0.02

2.3 ± 0.1

Bcl-XL (#42)

6

0.8 ± 0.2

0.7 ± 0.05

85.4 ± 0.6

BclX-Δt (#1)

5

0.6 ± 0.1

0.5 ± 0.01

8.7 ± 1.7

BclX-acta (#6)

4

0.3 ± 0.1

0.2 ± 0.02

97.8 ± 1.5

BclX-cb5 (#24)

5

0.6 ± 0.1

0.6 ± 0.1

32.3 ± 1.8