Figure 1
Prevention of apoptosis by Bcl-2 and Bcl-XL in MCF-7 cells. Cell lysates (20 μg of protein) of vector control (neo) and transfected MCF-7 cells expressing Bcl-2 or Bcl-XL (as indicated) were separated by SDS-PAGE, electroblotted and visualized using antibodies for PARP, actin or caspase 8 as indicated. Untreated vector transfected cells (-) as a control for expression level in untreated cells. Expression of Bcl-2 and Bcl-XL does not change the amount of PARP in untreated cells. Cells were exposed to 29 ng/ml TNFα and 10 ng/ml cycloheximide (TNF) (30 h), 400 nM thapsigargin (TG) (60 h), 70 μMC2-ceramide (C2) (20 h), or 10 μM doxorubicin (Dox) (24 h) as indicated above the lanes. The migration of the cleavage products of PARP and caspase 8 are indicated to the left of the bands by arrows. The migration positions of molecular weight markers are indicated in kDa to the left of the panels.