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Table 1 Summary of FISH, MLPA and RT-PCR results for FHIT on the five ESCC cell lines.

From: A novel approach to simultaneously scan genes at fragile sites

Cell lines Modal chromosome number FISH 3p copy number* MLPA FHITexon dosage deletion RT-PCR
Control 46 2 Normal Normal transcript
SNO 47 2+ Homozygous deletion exon 5 Aberrant transcripts only
WHCO1 52 2+ 2 small Hemizygous deletion exons: 1 to 10 Normal transcript and weak expression
Aberrant transcript
WHCO3 51 2+ Normal Normal transcript
Weak expression
WHCO5 117 4 Hemizygous deletion exons 4 and 5 Aberrant transcript only and weak expression
WHCO6 47 2 Hemizygous deletions exons 1, 3, 4, 5, 7, 10. (preferential 4 and 5) Aberrant transcript only and weak expression
  1. *: A sign + in the FISH column refers to additional 3p copy number full or partial.