A novel approach to simultaneously scan genes at fragile sites

BMC Cancer

Table 1 Summary of FISH, MLPA and RT-PCR results for FHIT on the five ESCC cell lines.

Cell lines	Modal chromosome number	FISH 3p copy number*	MLPA *FHIT*exon dosage deletion	RT-PCR
Control	46	2	Normal	Normal transcript
SNO	47	2+	Homozygous deletion exon 5	Aberrant transcripts only
WHCO1	52	2+ 2 small	Hemizygous deletion exons: 1 to 10	Normal transcript and weak expression Aberrant transcript
WHCO3	51	2+	Normal	Normal transcript Weak expression
WHCO5	117	4	Hemizygous deletion exons 4 and 5	Aberrant transcript only and weak expression
WHCO6	47	2	Hemizygous deletions exons 1, 3, 4, 5, 7, 10. (preferential 4 and 5)	Aberrant transcript only and weak expression

*: A sign + in the FISH column refers to additional 3p copy number full or partial.

ISSN: 1471-2407