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Figure 5 | BMC Cancer

Figure 5

From: A novel role for 3, 4-dichloropropionanilide (DCPA) in the inhibition of prostate cancer cell migration, proliferation, and hypoxia-inducible factor 1alpha expression

Figure 5

Effect of DCPA on HIF-1α protein stability. A. PC-3 cells were cultured in serum-free medium for 24 h, followed by the incubation with 10% fetal bovine serum for 5 h. Then the cells were treated with solvent alone (CHX, top panel) or 150 μM DCPA (CHX+DCPA, bottom panel) for 1 h, followed by the incubation with 100 μM cycloheximide for 0 to 16 min. Cellular lysates were subjected to immunoblotting using antibodies against HIF-1α and HIF-1β. B. The intensity of HIF-1α protein signals obtained above was quantified from three replicate experiments using EagleSight densitometry software (Version 3.21; Stratagene). The densitometry data were the ratio of HIF-1α/HIF-1β, and normalized to that of the control (0 min).

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