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Figure 4 | BMC Cancer

Figure 4

From: A novel role for 3, 4-dichloropropionanilide (DCPA) in the inhibition of prostate cancer cell migration, proliferation, and hypoxia-inducible factor 1alpha expression

Figure 4

DCPA specifically inhibits expression of HIF-1α induced by insulin and IGF-I. DU145 (A) and PC-3 (B) cells were cultured in the basal medium without serum in the presence of 0, 75 or 150 μM DCPA for 24 h, followed by the incubation with (+) or without (-) 200 nM insulin for 6 h. The total cellular proteins were prepared from the cells, and used for the analysis of HIF-1α and HIF-1β protein expression as described above. DU145 (C) and PC-3 (D) cells were cultured in the basal medium without serum as above, followed by the incubation in the presence (+) or absence (-) of 2 nM IGF-I for 6 h. The HIF-1α and HIF-1β protein expression from the cells was analyzed by immunoblotting.

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