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Figure 3 | BMC Cancer

Figure 3

From: A novel role for 3, 4-dichloropropionanilide (DCPA) in the inhibition of prostate cancer cell migration, proliferation, and hypoxia-inducible factor 1alpha expression

Figure 3

Expression of HIF-1α was specifically induced by the addition of serum, and inhibited by the presence of DCPA. DU145 (A) and PC-3 (B) cells were cultured in serum-free MEM basal medium with Earle's salt in 0, 75, or 150 μM DCPA for 24 h, followed by the incubation with or without 10% fetal bovine serum for 6 h. Total cellular protein extracts were prepared from these cells, and aliquots of the extracts (30 μg) were used for the immunoblotting analysis using antibodies against HIF-1α and HIF-1β.

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