Figure 1

Migration of prostate cancer cells was inhibited by DCPA. Prostate cancer cells, DU145 (A) and PC-3 (B) cells, were cultured in serum-free medium in the presence (+) or absence (-) of 150 μM DCPA for 18 h as indicated. The cell migration assay was performed using Transwell migration chambers coated with 10 μg/ml collagen in the migration buffer with or without 10% fetal bovine serum for 6 h at 37°C as described in Materials and Methods. The migrated cells were counted, and data were expressed as mean of cell counts from replicate experiments. Bars, SD; * indicates significant difference when compared to cell migration without DCPA (P < 0.01). C. Viability of the prostate cancer cells was not affected by DCPA. The cells were cultured in the absence or presence of DCPA for 24 h, and cell viability assay was performed as described in Materials and Methods. The results were expressed as the mean percentage of cells capable of excluding trypan blue. These experiments were repeated at least twice. Bars, SD.