(Large gel, left side) TRAP mRNA expression in commercially available cell lines and cultured cells from malignant effusions, as detected by RT-PCR. The breast cancer cell lines BT20 and MDA-MB-468, and 2 (P1 and P2) of 3 cell cultures derived from breast cancer patients with malignant pleural effusions show TRAP mRNA expression, whereas no TRAP mRNA is detectable in cells from patient P3. Ovarian cancer cell lines OAW42 and SKOV3 and cells cultured from the malignant ascites of ovarian cancer patients A1 – A3 show TRAP mRNA expression. No TRAP mRNA is detectable in the choriocarcinoma cell line JAR, unlike choriocarcinoma cell line JEG-3, which shows marked expression. (Small gel, right side) Detection of TRAP mRNA expression in malignant melanoma and stimulated dendritic cells by RT-PCR. Malignant melanoma cells BML and Mel2a show expression of TRAP mRNA, expression being stronger in the latter. As expected, stimulated dendritic cells show strong expression of TRAP mRNA.