Figure 6

Precipitation of HGF binding proteins from C4-2 and PC3 cells, using beads coated with HGF or BSA. (A) Met was precipitated in the PC3 cell line, while (B) in the C4-2 cell line two bands were precipitated using HGF coated beads but not with BSA control beads. The lower major band is HGF itself; the upper 100 kDa band was purified and sequenced. Western-blot analyses (C) and sequencing of the upper band confirmed it to be nucleolin (see table 2). (D) The importance of nucleolin to HGF-stimulated spreading on laminin substrata was apparent when antibodies to nucleolin reduced the effect of HGF (E). The cell spreading behaviors were quantified and presented as the mean of triplicate experiments. Statistically significant differences from the control were at P =< 0.005 (*).