Figure 2

CaP cells express full length CD164 mRNA. RT-PCR detection of CD164 expression by LNCaP C4-2B, LNCaP (L) and PC3 (P) cells under basal (-) and CXCL12 (+) stimulated conditions (CXCL12 200 ng/ml at 2 h). Primers F1-R1 were used to identify the CD164 coding exons 1-2, F2-R2 identify exons 2-3 and F3-R3 identify exons 2-5. After RT-PCR, the amplicons were identified using polyacrylamide gels. A molecular weight ladder is shown to the left of each figure that corresponds to differences in 100 base pairs. The data demonstrate that full length CD164 mRNA is express by CaP cells.