Effects of HDAC inhibitors on HEX expression and nuclear localization in MCF-7 cells. Cells were treated for 30 hours with NaB (3 mM) and TSA (300 nM). Then, HEX mRNA levels were evaluated by real-time PCR and HEX localization by immunohistochemistry. Panel A: effect of NaB and TSA on HEX mRNA levels. Panle B: effect of NaB and TSA on the HEX diffuse nuclear localization. In both panels each bar represents the mean value ± standard deviation of three independent measures. In both panels, according the paired Student's T test, the differences between treated and untreated cells were highly significant (at least: p < 0.01).