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Table 3 Polyamine levels, enzymatic activities and genes expression in neoplastic colorectal tissue with and without K-ras mutation.

From: Peroxisome proliferator-activated receptor gamma and spermidine/spermine N1-acetyltransferase gene expressions are significantly correlated in human colorectal cancer

  Mutated K-ras n = 12 Non mutated K-ras n = 28 p
Putrescine 64.4 ± 14.7 41.0 ± 14.5 0.039
Spermidine 376.1 ± 49.2 248.8 ± 26.9 0.021
Spermine 652.2 ± 48.6 455.5 ± 55.99 0.020
Total polyamines 1093.0 ± 98.0 745.3 ± 85.5 0.012
ODC 525.1 ± 157.6 92.9 ± 26.5 0.002
SSAT 43.1 ± 5.6 25.92 ± 4.3 0.035
ODC mRNA 21.3 ± 4.4 8.79 ± 1.8 0.020
SSAT mRNA 18.0 ± 3.9 7.3 ± 1.4 0.011
PPARγ mRNA 2.9 ± 1.1 0.78 ± 0.2 0.032
  1. Polyamines are reported as nmol/g wet tissue. ODC activity is expressed as pmol 14CO2/h/mg of protein. SSAT activity is expressed as pmol N1-[14C] acetylspermidine/min/mg of protein. The relative mRNA levels are expressed as target gene/β-actin ratio. All data are presented as mean ± SEM (Mann Whitney test).