Steps | Time | Temperature for the various SNPs | ||||||
---|---|---|---|---|---|---|---|---|
 |  | IL1A +4845 | IL1B -511 | IL1B +3954 | IL1RN +2018 | IL4R +1902 | IL6 -174 | IL10 -1082 |
1 | 2 minutes | 50°C | 50°C | 50°C | 50°C | 50°C | 50°C | 50°C |
2 | 10 minutes | 95°C | 95°C | 95°C | 95°C | 95°C | 95°C | 95°C |
3 | 15 seconds | 95°C | 95°C | 95°C | 95°C | 95°C | 95°C | 95°C |
4 | 1 minutes | 59°C | 59°C | 61°C | 64°C | 61°C | 62°C | 62°C |
5 | 40 times | Go to step 3 | Go to step 3 | Go to step 3 | Go to step 3 | Go to step 3 | Go to step 3 | Go to step 3 |
6 | Hold | 15°C | 15°C | 15°C | 15°C | 15°C | 15°C | 15°C |