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Table 3 Cycling conditions for the PCRs for the different polymorphisms

From: Interleukin gene polymorphisms and breast cancer: a case control study and systematic literature review

Steps Time Temperature for the various SNPs
   IL1A +4845 IL1B -511 IL1B +3954 IL1RN +2018 IL4R +1902 IL6 -174 IL10 -1082
1 2 minutes 50°C 50°C 50°C 50°C 50°C 50°C 50°C
2 10 minutes 95°C 95°C 95°C 95°C 95°C 95°C 95°C
3 15 seconds 95°C 95°C 95°C 95°C 95°C 95°C 95°C
4 1 minutes 59°C 59°C 61°C 64°C 61°C 62°C 62°C
5 40 times Go to step 3 Go to step 3 Go to step 3 Go to step 3 Go to step 3 Go to step 3 Go to step 3
6 Hold 15°C 15°C 15°C 15°C 15°C 15°C 15°C