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Table 3 Cycling conditions for the PCRs for the different polymorphisms

From: Interleukin gene polymorphisms and breast cancer: a case control study and systematic literature review

Steps

Time

Temperature for the various SNPs

  

IL1A +4845

IL1B -511

IL1B +3954

IL1RN +2018

IL4R +1902

IL6 -174

IL10 -1082

1

2 minutes

50°C

50°C

50°C

50°C

50°C

50°C

50°C

2

10 minutes

95°C

95°C

95°C

95°C

95°C

95°C

95°C

3

15 seconds

95°C

95°C

95°C

95°C

95°C

95°C

95°C

4

1 minutes

59°C

59°C

61°C

64°C

61°C

62°C

62°C

5

40 times

Go to step 3

Go to step 3

Go to step 3

Go to step 3

Go to step 3

Go to step 3

Go to step 3

6

Hold

15°C

15°C

15°C

15°C

15°C

15°C

15°C