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Table 2 Final concentration of the different constituents of the PCR mixture

From: Interleukin gene polymorphisms and breast cancer: a case control study and systematic literature review

PCR constituents Final concentrations for the various SNPs
  IL1A +4845 IL1B -511 IL1B +3954 IL1RN +2018 IL4R +1902 IL6 -174 IL10 -1082
Taqman mastermix (2X) 1X 1X 1X 1X 1X 1X 1X
Forward primer (10 μM) 500 nM 100 nM 300 nM 250 nM 50 nM 50 nM 50 nM
Reverse primer (10 μM) 500 nM 100 nM 300 nM 250 nM 500 nM 50 nM 300 nM
FAM probe (5 μM) 50 nM 50 nM 50 nM 30 nM 30 nM 30 nM 50 nM
TET probe (5 μM) 100 nM 100 nM 75 nM 150 nM 120 nM 60 nM 150 nM
Template (20 ng/μl) 0.8 ng/μl 0.8 ng/μl 0.8 ng/μl 0.8 ng/μl 0.8 ng/μl 0.8 ng/μl 0.8 ng/μl
  1. Taqman mastermix: Universal PCR mastermix (PE Biosystems) containing MgCl2, dNTPs, Taq polymerase, optimised buffer components and Rox reference dye; FAM probe: 6-carboxy-fluorescein-labelled probe; TET: 6-carboxy-4,7,2',7'-tetrechlorofluorescein-labelled probe.
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BMC Cancer

ISSN: 1471-2407

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