Advertisement
Table 2 Final concentration of the different constituents of the PCR mixture
| PCR constituents | Final concentrations for the various SNPs | ||||||
|---|---|---|---|---|---|---|---|
| IL1A +4845 | IL1B -511 | IL1B +3954 | IL1RN +2018 | IL4R +1902 | IL6 -174 | IL10 -1082 | |
| Taqman mastermix (2X) | 1X | 1X | 1X | 1X | 1X | 1X | 1X |
| Forward primer (10 μM) | 500 nM | 100 nM | 300 nM | 250 nM | 50 nM | 50 nM | 50 nM |
| Reverse primer (10 μM) | 500 nM | 100 nM | 300 nM | 250 nM | 500 nM | 50 nM | 300 nM |
| FAM probe (5 μM) | 50 nM | 50 nM | 50 nM | 30 nM | 30 nM | 30 nM | 50 nM |
| TET probe (5 μM) | 100 nM | 100 nM | 75 nM | 150 nM | 120 nM | 60 nM | 150 nM |
| Template (20 ng/μl) | 0.8 ng/μl | 0.8 ng/μl | 0.8 ng/μl | 0.8 ng/μl | 0.8 ng/μl | 0.8 ng/μl | 0.8 ng/μl |
