PCR constituents
|
Final concentrations for the various SNPs
|
---|
|
IL1A +4845
|
IL1B -511
|
IL1B +3954
|
IL1RN +2018
|
IL4R +1902
|
IL6 -174
|
IL10 -1082
|
---|
Taqman mastermix (2X)
|
1X
|
1X
|
1X
|
1X
|
1X
|
1X
|
1X
|
Forward primer (10 μM)
|
500 nM
|
100 nM
|
300 nM
|
250 nM
|
50 nM
|
50 nM
|
50 nM
|
Reverse primer (10 μM)
|
500 nM
|
100 nM
|
300 nM
|
250 nM
|
500 nM
|
50 nM
|
300 nM
|
FAM probe (5 μM)
|
50 nM
|
50 nM
|
50 nM
|
30 nM
|
30 nM
|
30 nM
|
50 nM
|
TET probe (5 μM)
|
100 nM
|
100 nM
|
75 nM
|
150 nM
|
120 nM
|
60 nM
|
150 nM
|
Template (20 ng/μl)
|
0.8 ng/μl
|
0.8 ng/μl
|
0.8 ng/μl
|
0.8 ng/μl
|
0.8 ng/μl
|
0.8 ng/μl
|
0.8 ng/μl
|
- Taqman mastermix: Universal PCR mastermix (PE Biosystems) containing MgCl2, dNTPs, Taq polymerase, optimised buffer components and Rox reference dye; FAM probe: 6-carboxy-fluorescein-labelled probe; TET: 6-carboxy-4,7,2',7'-tetrechlorofluorescein-labelled probe.