DHPLC diagram and p53 sequencing analysis. (a) DHPLC diagram. The carcinomatous component is represented in black while the sarcomatous component in red and the wild-type control sample in blue. The same genetic alteration in both tumour components is illustrated by the presence of an extra peak in the neoplastic specimens in comparison with the normal control. (b) Sequencing analysis showing p53 exon 8 mutation R282W (arrow).