Table 1 Effect of COX-2 Inhibitor and PGE₂ on MMP mRNA Expression. Expression of MMP mRNA in Hs578T cells in the presence of NS-398 with or without 1 μM PGE₂. Hs578T cells were cultured in the presence NS-398 with or without PGE₂ for 24 h. The relative quantitation of the gene expression of the MMP mRNA was calculated against an 18S rRNA standard. The number of copies of MMP mRNA per 10⁶ copies of 18S rRNA was determined by real time-PCR according to the methods of Singh et al [25]. The real-time PCR analysis was capable of detecting >5 copies of mRNA. Hence, copy numbers <5 were below detection (BD) limits. Significance (n = 4, *p < 0.05) was determined by comparing the treated Hs578T cells to the untreated control. ^#The data in this column has an n = 2 and is listed to show the direction of the effect of PGE₂.

From: Inhibition of cyclooxygenase-2 decreases breast cancer cell motility, invasion and matrix metalloproteinase expression