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Table 1 Effect of COX-2 Inhibitor and PGE2 on MMP mRNA Expression. Expression of MMP mRNA in Hs578T cells in the presence of NS-398 with or without 1 μM PGE2. Hs578T cells were cultured in the presence NS-398 with or without PGE2 for 24 h. The relative quantitation of the gene expression of the MMP mRNA was calculated against an 18S rRNA standard. The number of copies of MMP mRNA per 106 copies of 18S rRNA was determined by real time-PCR according to the methods of Singh et al [25]. The real-time PCR analysis was capable of detecting >5 copies of mRNA. Hence, copy numbers <5 were below detection (BD) limits. Significance (n = 4, *p < 0.05) was determined by comparing the treated Hs578T cells to the untreated control. #The data in this column has an n = 2 and is listed to show the direction of the effect of PGE2.

From: Inhibition of cyclooxygenase-2 decreases breast cancer cell motility, invasion and matrix metalloproteinase expression

Type of MMP NS-398 (μM) NS-398/PGE2 (μM) #
  Control 1 10 100 1.0/1.0
MMP-1 1.04 × 102 BD BD BD 3.58 × 103
MMP-2 2.09 × 105 3.93 × 103* 3.87 × 102* 1.06 × 103* 3.11 × 107
MMP-3 3.42 × 105 5.73 × 102* 28.0* 1.08 × 102* 3.26 × 1013
MMP-9 16.4 BD BD BD 1.06 × 105
MMP-10 BD BD BD BD 38.4
MMP-11 8.31 × 103 6.92 BD BD 1.85 × 109
MMP-13 3.93 × 102 57.9 11.7 4.83 1.70 × 106
MMP-14 BD BD BD BD 4.53 × 104