The effect of NS-398 on metalloproteinase release in culture media by Hs578T cells. The cells were cultured in the presence or absence of increasing doses of NS-398 for 24 h. The levels of the pro and active form of MMPs-1, 2, 3, 9, 10 and 13 were measured in the culture media of treated cells by EIA. A) MMP-1 B) MMP-2 C) MMP-3 D) MMP-13. MMP-9 and MMP-10 were not detected in Hs578T media. Values were significant (*p < 0.05) when compared to untreated controls (0 μM NS-398).