The effect of COX-2 inhibition on the migration of MDA-231 cells. A) Cells (1 × 105) were placed in the upper chamber inserts with or without NS-398 in serum-free DMEM and allowed to migrate for 24 h. FCM was used as a chemoattractant to stimulate the migration of the cells. B) Wound migration assay. Confluent MDA-231 cells cultured in six well dishes were wounded with a sterile pipette tip and then incubated with or without Niflumic Acid for 24 h. Photographs were taken with a phase contrast microscope and measurements with Adobe photoshop 6.0. Values were significant (*p < 0.05, **p < 0.01) when compared to untreated controls (0 μM NA or NS-398).