Representative autoradiographs of semiquantitative methylation analysis (A) and the number and position of the methylated CpGs identified by the sequencing of common PCR DNA (B). (A) The 5'-transitional CpG sites generated a wide range of methylated and unmethylated PCR band intensities from the bisulfite-modified DNA from Case 1, with a high-level chromosomal loss, and Case 26, with a low-level chromosomal loss. The lanes marked U and M indicate the PCR bands of the unmethylation and methylation primer sets, respectively. The level of the methylated CpGs calculated from a standard MSP curve is indicated below the lanes. (B) For the same cases, the content of methylated CpGs was estimated from the common PCR DNA containing both the methylated and unmethylated CpGs. Ten common PCR clones per DNA sample were sequenced. The content of methylated CpGs is indicated as a percentage on the left side of each CpG methylation map. The different methylation results between the MSP bands and the common PCR DNAs are indicated by an asterisk.