In vitro and in vivo MMP gene expression localisation by In Situ-RT-PCR in cell culture and paraffin embedded human breast cancer cell line xenografts

Table 2 Summary of IS-RT-PCR results obtained for expression of β-actin, MT1-MMP, MMP-1 and MMP-3 in HBC cell line derived xenograft tissues. The intensity is a value from zero to four (0+ to 4+) based on the overall level of signal intensity observed throughout the entire section.

Cell Line	β-actin	MT1-MMP	MMP-1	MMP-3
MCF-7	Tumour 2+	Tumour 1+	Tumour 1+	Tumour 1+
	Stroma 1+	Stroma 1+	Stroma 1+	Stroma 1+
MDA-MB-231	Tumour 2+	Tumour 3+	Tumour 0+	Tumour 1+
Spleen	Stroma 2+	Stroma 0+	Stroma 0+	Stroma 0+
MDA-MB-231	Tumour 2+	Tumour 2+	Tumour 2+	Tumour 1+
Mesenteric	Stroma 2+	Stroma 1+	Stroma 2+	Stroma 1+
MDA-MB-435	Tumour 2+	Tumour 2+	Tumour 2+	Tumour 3+
	Stroma 2+	Stroma 0+	Stroma 0+	Stroma 0+
Hs578T	Tumour 1+	Tumour 1+	Tumour 1+	Tumour 1+
	Stroma 1+	Stroma 1+	Stroma 0+	Stroma 2+

ISSN: 1471-2407