Figure 5
Densitometric analysis of western blots (WB) of cleaved PARP (89 kDa; A), caspase 3 (32 kDa; B) and cytosolic cytochrome-c (34 kDa; C) and WB images of cleaved PARP, caspase-3 and cytosolic cytochrome-c in the spongy trophoblast of control, tumour-bearing and ascitic fluid-injected rats on the 16th, 19th and 21st day of pregnancy. Cleaved PARP (antibody dilution 1:500), caspase-3 and cytochrome-c (antibody dilution 1:1000) were detected with polyclonal antibodies from Santa Cruz Biotechnology and enhanced chemiluminescence. For details see Materials and Methods. The band intensities (expressed in arbitrary densitometric units) were analysed using Gel-Pro Analyser software. Actin was used as an internal control for protein loading and was detected with an antibody to mouse actin. The WB images are representative of eight separate blots. Groups: C – control, W – tumour-bearing, and A – ascitic fluid-injected rats. The results are expressed as the mean ± SEM of at least six rats per group. Different superscript letters indicate significant differences (P < 0.05) within each column.