Figure 2

Immunohistochemical detection of cleaved PARP in placental tissue at different stages of pregnancy (days 16, 19 and 21). Cleaved PARP was detected using a polyclonal antibody (Santa Cruz; diluted 1:100) followed by incubation with a biotinylated anti-goat antibody (1:500) and reaction with DAB in the presence of H₂O₂. The tissue sections are from the placental junctional zone, which contains cytotrophoblastic cells (c) and trophoblastic giant cells (gc). The arrows indicate nuclei with immunostaining for cleaved PARP. Groups: C – control rats, corresponding to images A, B and C on the 16^th, 19^th and 21^st day, respectively, W – tumour-bearing rats, corresponding to images D, E and F on the 16^th, 19^th and 21^st day, respectively, and A – rats injected with ascitic fluid, corresponding to images G, H and I on the 16^th, 19^th and 21^st day, respectively. Image J is a negative control. Magnification – 100×. Horizontal bar = 100 μm.