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Table 1 Primers used in the PCR study and medians (range) of mRNA expression of hBDs in patients with basal cell carcinoma and healthy controls.

From: Pattern of mRNA expression of β-defensins in basal cell carcinoma

Primer Sequence
hBD-1 F 5'-AGATGGCCTCAGGTGGTAACTTT-3'; R 5'-GGGCAGGCAGAATAGAGACATT-3'
hBD-2 F 5'-TGATGCCTCTTCCAGGTGTTT-3'; R 5'- GGATGACATATGGCTCCACTCTT-3'
hBD-3 F 5'-TCCATTATCTTCTGTTTGCTTTGC-3'; R 5'-TTCTGTAATGTGTTTATGATTCCTCCAT-3'
GAPDH F 5'-CCTCAACTACATGGTTTACATGTTCC-3'; R 5'-ATGGGATTTCCATTGATGACAAG-3'
Medians and range of mRNA expression
  lesional non-lesional healthy controls
hBD-1 0.154 (0.002 – 0.634) 0.389 (0.111 – 0.941) 0.431 (0.161 – 0.981)
hBD-2 0.053 (0.000 – 0.974) 0.008 (0.001 – 0.162) 0.004 (0.000 – 0.178)
hBD-3 0.057 (0.002 – 0.915) 0.048 (0.006 – 0.263) 0.041 (0.004 – 0.541)
  1. hBD, human β-defensin; GAPDH, Glyseraldehyde-3-phosphate dehydrogenase