Detection of proteins hyperphosphorylated after treatment of PWBC with GSAO using an Akt substrate-directed antibody. Cells were incubated for 24 hwith GSAO or GSCA as indicated and analysed with an antibody made to recognise multiple Akt substrate proteins in their Akt-phosphorylated forms (R/K-x-R/K-x-x-pS/pT motif, Cell Signalling Technology 9611). A Massive effect of GSAO on phosphoproteins detected with the Akt substrate phospho-antibody using protein extracts from low serum cultured PWBC. The control compound GSCA does not detectably change the basal phospho-protein pattern. B Effects of 15 μM GSAO on high serum culturedPWBC. Arrows indicate bands that are hyperphosphorylated inGSAO-treated cells.