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Figure 5 | BMC Cancer

Figure 5

From: Molecular profiling of signalling proteins for effects induced by the anti-cancer compound GSAO with 400 antibodies

Figure 5

GSAO induces phosphorylation of a 100 kDa protein which is recognised by an antibody directed against PRK1/PKNα pThr778. PWBC were treated with GSCA or GSAO where indicated for 24 h and proteins analysed with anti-PRK1/PKNα pThr778. A Effect of 50 μM GSAOvs. GSCA on a 100 kDa protein recognised by anti-PRK1/PKNα pThr 778. A prominent hyperphosphorylation of the 100 kDa protein is visible, while a signal of ca. 120 kDa, the size expected for PRK1/PKNα, remains unchanged. The 100 kDa band may correspond to the PRK1/PKNα relative PKNβ, but this can only be verified after the generation of PKNβ-specific antibodies. B GSAO dose – response in low serum cultured PWBC. Hyperphosphorylation of the 100 kDa band is visible from 15 μM of GSAO with the pThr778-specific PRK1/PKNα antibody (upper panel) while total protein levels of PRK1/PKNα remain unchanged (lower panel). A mobility shift of PRK1/PKNα is also visible with this antibody at higher GSAO concentrations, possibly indicating a PRK1/PKNα phosphorylation at a site different from pThr778. C Effect of GSAO on the 100 kDa band in high serum cultured PWBC. A moderate increase of phosphorylation at the pThr778-related epitope is visible at 50 μM GSAO.

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