GSAO effects Erk, but not p90Rsk or Jnk phosphorylation in PWBC high serum culture. PWBC were cultured in 100% donor serum and treated with GSAO or GSCA for 24 h as indicated.A Phosphorylation of Erk2 on the Thr202/Tyr204 epitope is visible at 50 μM GSAO (upper panel). Total Erk protein levels remain unchanged after GSAO treatment for 24 h (lower panel). B Phosphorylation of Ser380 on p90Rsk is marginally increased at best in high serum cultured PWBC. C Phosphorylation of the Thr183/Tyr185 epitope on Jnks is marginally increased at best in high serum cultured PWBC.