Figure 4

KINE and CON cells were tested for strength of adhesion to substrate. Adhesion assays were conducted by plating cells on uncoated tissue culture dishes followed by trypsinization for 10 min using various concentrations of tryspin. T-test at 5% confidence levels showed significant difference at lower concentrations of trypsin used (asterisks), indicating greater sensitivity to trypsinization in KINE compared to CON cells (n = 3). Conversely, the resistance of CON cells to trypsinization suggests stronger adhesion to substrate.