Figure 3

Adhesion structures of CON and KINE cells. Panels A & D show paxillin-y118 localization. Panels B & E show actin staining and Panels C & F show merged channels for paxillin-y118 and actin. Cells grown in complete medium were fixed and processed for the immunolocalization of paxillin-y118. CON cells stained for paxillin-y118 at focal adhesions at the tip of the uropod (arrowhead) and the leading edge (arrows) (A, C). The focal adhesions at the leading edge were connected to internal actin cables (B, C and inset). In KINE cells, however, focal adhesions were absent from the leading edge but less distinct focal complexes are present within the lamellae (arrowheads) (D, F). The leading edge of KINE cells show many actin-rich ruffles (arrows) (E, F) (Bars = 5 microns).