Figure 4
In vivo angiostatin formation in healthy mice after treatment with tPA and/or D-penicillamine. In vivo angiostatin generation was induced by administration of tPA (two i.v. injections, on day 3 and 7) and/or D-penicillamine (i.p. injection, twice daily, starting prior to the first tPA injection) to healthy BALB/c mice. Plasma samples were collected prior to the tPA injection on day 7 (t = 0), and 15 or 120 minutes after tPA injection. After purification by lysine sepharose affinity chromatography, samples were analyzed on western blot. Note a complete conversion of plasminogen to plasmin, and the formation of angiostatin in mice receiving tPA and D-penicillamine, 15 minutes after tPA injection.