Figure 2From: Angiostatin generating capacity and anti-tumour effects of D-penicillamine and plasminogen activators Example of in vitro angiostatin generation by tissue-type plasminogen activator and FSDs. A mixture of murine serum, tPA (2 μg/ml) and a FSD (D-penicillamine, L-cysteine, N-acetyl-L-cysteine (NAC), reduced glutathione, or captopril) was incubated for 0, 2, 4.5 or 20 hours. Conversion of plasminogen to plasmin (left panel) and plasmin to angiostatin (right panel) were scored separately on a semi-quantitative scale. D-penicillamine was the most effective FSD for generation of plasmin and angiostatin. : D-penicillamine; : L-cysteine; : N-acetyl L-ysteine; : reduced glutathione; : captopril.Back to article page