Figure 5
Expression of cFLIP-L or resistance to death receptor ligands do not interfere with Ukrain-induced apoptosis Jurkat J16 control cells, cFLIP-L expressing Jurkat cells and CD95/TRAIL resistant Jurkat cells were treated for 24 h with medium, 10 ng/ml TRAIL or 10 μg/ml Ukrain. Apoptosis was then quantified by fluorescence microscopy upon Hoechst33342-staining and counting of cells with apoptotic morphology (means ± SD; n = 3). One-way ANOVA was performed using GraphPad InStat version 3.00 for Windows 95, GraphPad Software, San Diego California USA, http://www.graphpad.com.