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Figure 3 | BMC Cancer

Figure 3

From: Proapoptotic activity of Ukrain is based on Chelidonium majusL. alkaloids and mediated via a mitochondrial death pathway

Figure 3

(a) Ukrain-induced apoptosis depends on caspase-activation. Jurkat A3 cells were pre-incubated for 2 h with medium supplemented with 30 μM of the pan-caspase-inhibitor zVAD-fmk or left untreated. Subsequently, cells were treated for 24 h with medium or 10 μg/ml Ukrain. Apoptosis was then quantified by counting of the cells with apoptotic nuclear morphology (left panel). Data show means ± SD, n = 3. Paired t-test was performed using GraphPad InStat version 3.00 for Windows 95, GraphPad Software, San Diego California USA, Micrographs showing reduced induction of apoptotic nuclear morphology upon combined treatment are depicted in the right panel. (b) Ukrain exerts concentration- and time-dependent cytotoxic effects on Jurkat T-lymphoma cells. Jurkat Vector cells were treated for 24 h with medium, 5, 10 and 50 μg/ml Ukrain (left panel) or for 0, 12, 24 or 48 h with 10 μg/ml Ukrain (right panel). The number of living and dead cells was then quantified by light microscopy using Trypan Blue Exclusion. Data show means ± SD, n = 3.

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