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Table 1 Oligonucleotides and plasmids used in this study.

From: Analysis of folylpoly-γ-glutamate synthetase gene expression in human B-precursor ALL and T-lineage ALL cells

PCR products/Plasmids

Forward and Reverse primers/Description

1163 bp

exon15-53F (5'-CGTTCTCCCCATGAACTTACA)

 

exon15-1215R (5'-AGGGTCTAGGCTGCAAGAAAG)

1374 bp

intron0F-141 (5'-ACCCCAGCAGTGTGTGAAGAG)

 

intron1R-6537 (5'-CCCACCCCCAGATCAATACTC)

1926 bp

1exonF (5'-GGGGGCGCCGGGACTATGTCG)

 

15exonR-c1934 (5'-TCTCCCGGCCTCCCATCCCAA)

2158 bp

46714F (5'-AGAAGTTCTGGTGGGAAGGAA)

 

48871R (5'-CAGACCCTGGACTAGATGCTG)

2299 bp

48538F (5'-TAAGTGGGGGATGAGTCCAG)

 

50836R (5'-CCCAGATTCCAGCATCCTAA)

2628 bp

17595F (5'-GGCGCAGAGGCTAAGAATAAG)

 

20202R2 (5'-CCCCCAGCCTCCAGGATGTTC)

2881 bp

exon15-53F (5'-CGTTCTCCCCATGAACTTACA)

 

exon15-2933R (5'-CCACACAGGAGTCAGGAATGT)

3588 bp

F8256K (5'-GAGAGAGGTACCCCCGTGACTCCTGGTGGCTGC)

 

R11843K (5'-AATTCGGTACCCCTGGTCACCGGGTTCTCCTA)

4709 bp

UA1F1979 (5'-CGCACACACCGCCAACTGTTC)

 

UA1R6667 (5'-CCTGGTCACCGGGTTCTCCTA)

4970 bp

UAP-15639 (5'-GAGTGGCCCTTATGTACCGAC)

 

1R-20591 (5'-GGGCAACCGGCTCTTGAC)

ATGc

QC20060F (5'-CGCAGGAGCCGAGCTCGGAGTACCAGGTAT)

 

QC20060R (5'-ATACCTGGTACTCCGAGCTCGGCTCCTGCG)

ATGm

QC19934F (5'-GCGCCGGGACTAGTTCGCGGGCGCGG)

 

QC19934R (5'-CCGCGCCCGCGAACTAGTCCCGGCGC)

NFY-868

NFY-868F (5'-GACGCTGCGCTGAAAAGCTGGGGGCGGGGC)

 

NFY-868R (5'-GCCCCGCCCCCAGCTTTTCAGCGCAGCGTC)

Ebox-952

E47-952F (5'-GGGCGCGGAGCCATTCGCGCGCCGCTCTATTC)

 

E47-952R (5'GAATAGAGCGGCGCGCGAATGGCTCCGCGCCC)

139 bp (MSP)2

M19571F 5'-GTAGAGATTGAGGGTTGTTGATTC

 

M19709R 5'-CGAAACCAAATTTATAAATATACGCT

142 bp (MSP)2

U19571F 5'-GTAGAGATTGAGGGTTGTTGATTT

 

U19712R 5'-CCTCAAAACCAAATTTATAAATATCACT

pCR1163

1163 bp PCR fragment from NALM6 cloned into pCR2.1 TOPO

pCR1374

1374 bp PCR fragment from BM cloned into pCR2.1-TOPO

pCR2158

2158 bp PCR fragment from NALM6 cloned into pCR2.1-TOPO

pCR2299

2299 bp PCR fragment from NALM6 cloned into pCR2.1-TOPO

pCR2628

2628 bp PCR fragment from BAC RCPI-11 cloned into pCR2.1-TOPO

pCR2881

2881 bp PCR fragment from NALM6 cloned into pCR2.1-TOPO

pCR4.7A1b

4709 bp PCR fragment from BM cloned in pCR2.1-TOPO

pCR4970

4970 bp PCR fragment from BAC RCPI-11 cloned into pCR2.1-TOPO

pFPGS-cDNA

1926 bp PCR from NALM6 cloned into pCR2.1-TOPO

pGL1163-2628

1163 bp Acc65I fragment PCR from pCR1163 cloned in pGL2628ATGm/c

pGL1374

1374 bp HindIII PCR fragment from pCR1374 cloned into pGL3

pGL2158-2628

2158 bp Acc65I PCR fragment from pCR2158 cloned in pGL2628ATGm/c

pGL2256

2316 bp HindIII fragment from pCR4.7-A1b cloned into pGL3

pGL2299-2628

2299 bp Acc65I PCR fragment from pCR2299 cloned in pGL2628ATGm/c

pGL2628

2628 bp SacI-EcoRV fragment from pCR2628 cloned into pGL3

pGL2628-ATGc

cytosolic ATG mutagenized to TCG (ATGc) in pGL2628

pGL2628-ATG

mitochondrial ATG mutagenized to AGT (ATGm) in pGL2628

pGL2628-ATGm/c

cytosolic ATG mutagenized to TCG (ATGc) in pGL2628-ATGm

pGL2881-2628

2881 bp Acc65I PCR fragment from pCR2881 cloned in pGL2628ATGm/c

pGL3588-2628

3588 bp Acc65I PCR fragment from pCR4.7A1b in pGL2628ATGm/c

pGL4689

4708 bp EcoRV-BamHI fragment from pCR4970 cloned into pGL3

pGL868NFY-ATGm/c

NFY-868 mutation in pGL2628-ATGm/c

pGL952NFY-ATGm/c

Ebox-952 mutation in pGL2628-ATGm/c

  1. 1Nucleotides substituted are in bold italic. 2 MSP, Methyl-Specific PCR BM, normal human bone marrow genomic DNA.
  2. To construct pGL1163-2628, pGL2158-2628, pGL2299-2628, pGL2881-2628, and pGL3588-2628, a sequence (5'-AATTCGGTACC) containing the Acc65I restriction site (underlined) was added to the 5'-end of the oligonucleotide primers used for the PCR amplification.