Figure 3

FPGS promoter-luciferase constructs and activity in CCRF-CEM and NALM6 cells. Genetic organization of the FPGS promoter-luciferase constructs is shown with their normalized luciferase/β-galactosidase activity in CCRF-CEM and NALM6 cells. Horizontal lines represent fragments cloned into pGL3 (luc) vector. Size of the fragments is indicated. Pointed boxes represent exons, and arrows direction of gene expression. Exponential cultures of CCRF-CEM and NALM6 cells (5 × 10⁶) were transfected by nucleofection with equimolar concentration of pGL1374/pCMVβ and other FPGS promoter::luc constructs as described in Materials and Methods. Luciferase activity (RLU; relative light units) was initially subtracted from the level of luciferase activity detected with pGL3 (empty vector) and normalized with respect to the level of β-galactosidase activity (RLU). Each experiment was performed at least two times in triplicate. Bars represent mean ± SD. See details in Materials and methods.