Figure 4
The annexin V/propidium iodide double staining assay following 100 μM tocopherol treatment in HCT-116 cells (Panels A-D) for 24 hours and SW480 (Panels E-H) cells for 70 hours. These data show RRR-γ-tocopherol is superior to RRR-α-tocopherol at inducing apoptosis. The percentages in right quadrants represent percentage apoptosis over the blank and are an average of at least two independent trials.