Figure 5
Apoptosis induced by LACE. B9 cell line was untreated or treated with 250 ÎĽg/ml or 1 mg/ml of LACE for 96 h. Cells were double-stained with annexin V and 7AAD and analyzed by flow cytometry. PBLs were also analyzed and LACE extract did not produce apoptosis. All experiments were performed at least three times and gave similar results.