Effect of LACE on cyclins and CDKs. The cell lines AGS and JURKAT were cultured in alone medium or treated with LACE. a) Flow cytometric analysis was performed for the expression of cyclins D1 and E. The LACE extract produced a strong inhibition of expression of both cyclins. b) The western blot analyses showed that LACE produced down-regulation of cyclins D3 y A and CDK1/Cdc2, CDK2, CDK4, CDK6. The cyclin B expression was not modified.