Figure 2

Kinetic changes in the gene expression during mycoplasma-induced transformation. Total RNAs were prepared from C3H cells infected with M. fermentans for 1 week (wk), 11 weeks (wks) and 18 wks as well as from noninfected control cells (Ctrl). Radioactive cDNA probes prepared from these RNAs were hybridized to the Mouse Cancer 1.2 Arrays. Results were obtained by the autoradiography. Four autoradiographic pictures in Panel A represent the same areas on the arrays hybridized with cDNA probes prepared from control cells and cells infected with the mycoplasmas for 1 wk, 11 wks and 18 wks. Dots pointed by arrows are individual genes: a. Extracellular matrix protein 1; b. Procollagen III α1 subunit; c. T-complex protein 1 epsilon subunit and d. Fibrillin 1. Panel B is graphic depiction of kinetic changes of the expression of the above 4 genes during mycoplasma-induced transformation process. Scale on Y axis is the relative gene expression level and X axis is different stages of the mycoplasma induced transformation.