Figure 4

Localization of KIAA0101 predominantly in mitochondria. HCC SMMC 7721 Cells were seeded on slip for dual immunofluorescence analysis. Primary antibodies for cytochrome oxidase I and KIAA0101 were detected using FITC- and TRITC- conjugated secondary antibodies, respectively. Yellow fluorescence in the overlay image indicated the co-localization of the red fluorerscence of KIAA0101 with the green fluorescence of mitochonriion specific of cytochrome oxidase I.