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Table 2 Determination of IC50 values in an in vitro kinase assay. IC50 values (μM) for the inhibition of recombinant CK2, PKD and the purified CSN complex by the inhibitors of the curcumin-group, piceatannol as well as BTB00363, and of the emodin-group, BTB14431, JFD02836, SEW04213 and JFD03665 were determined. The two groups of inhibitors were created based on the different structures of the lead compounds (see Table 1). Kinase assays were performed as outlined in materials and methods. For comparison IC50 values of curcumin, resveratrol and emodin from earlier measurements [2] are shown.

From: Novel curcumin- and emodin-related compounds identified by in silico 2D/3D conformer screening induce apoptosis in tumor cells

Inhibitor IC50 CK2 (μM) IC50 PKD (μM) IC50 CSN (μM)
Curcumin-group
Curcurmin 11.8 4.1 2.6 *
Resveratrol 51.0 17.6 32.1 *
Piceatannol 2.5 0.5 1.7
BTB00363 332.7 117.7 188.6
Emodin-group
Emodin 22.7 94.5 4.4 *
BTB14431 6.4 68.9 21.0
JFD02836 19.9 39.7 54.1
SEW04213 37.9 185.9 4.8
JFD03665 5.9 154.9 19.8
  1. * [2]