Figure 6
From: Effects of alpha fetoprotein on escape of Bel 7402 cells from attack of lymphocytes
The effects of AFP on the expression of survivin protein. Jurkat T cells and Bel 7402 cells were co-cultured for 24 h and treated with AFP (20 mg/L), HSA (20 gm/L), AFP (20 mg/L) plus anti-AFP antibody (40 mg/L) and anti-AFP antibody (40 mg/L) for 36 h respectively. The expression of survivin was detected with Western blotting. Lane 1: control; Lane 2: cells separate-cultured cells treated with AFP; Lane 3: co-cultured cells; Lane 4: co-cultured cells treated with AFP; Lane 5: co-cultured cells treated with AFP and anti-AFP antibody; Lane 6: co-cultured cells treated with anti-AFP antibody. All images were representative of an experiment that was repeated three times.