Confocal assay for the expression of FasL on the membrane of co-cultured Bel 7402 cells. Bel 7402 cells and Jurkat T cells were co-cultured with AFP (20 mg/L) for 48 h. After removing Jurkat cells, Bel 7402 cells were incubated with rabbit anti-FasL antibody and secondary goat anti-rabbit IgG antibodies conjugated with FITC. The cells were observed using a confocal laser scanning microscope. Image A: separate-cultured Bel 7402 cell; B: separate-cultured Bel 7402 cells treated with AFP. Right images of A and B were taken under common microscope to observe the state of cells. C: co-cultured Bel 7402 cells without AFP treatment; D: co-cultured Bel 7402 cells treated with AFP. All images were representative of an experiment that was repeated three times.