Figure 3

Confocal assay for the expression of Fas on the membrane of co-cultured Bel 7402 cells. Bel 7402 cells and Jurkat T cells were co-cultured with AFP (20 mg/L), anti-AFP antibody (40 mg/L), and AFP (20 mg/L) plus anti-AFP antibody (40 mg/L) respectively for 48 h. After removing Jurkat cells, Bel 7402 cells were incubated with rabbit anti-Fas antibody and secondary goat anti-rabbit IgG antibodies conjugated with FITC. The cells were observed using a confocal laser scanning microscope. Image A: separate-cultured Bel 7402 cell; B: separate-cultured Bel 7402 cells treated with AFP; C: co-cultured Bel 7402 cells treated with AFP; D: co-cultured Bel 7402 cells treated with anti-AFP antibody. Right images from A to D were taken under common microscope to observe the state of cells. E: co-cultured Bel 7402 cells; F: co-cultured Bel 7402 cells treated with AFP and anti-AFP antibody. All images were representative of an experiment that was repeated three times.