Figure 2

The effects of AFP on the co-cultured cells. Bel 7402 and Jurkat cells were co-cultured for 24 h following treated with AFP (20 mg/L) (C), AFP (20 mg/L) plus anti-AFP antibody (40 mg/L) (D), anti-AFP antibody (40 mg/L) (E) and HSA (20 gm/L) (F) respectively. Graph bar A represents the viability of separate-cultured cells. Graph bar B represents co-cultured and no-treatment group. After 60 h, two cell lines were separated and the viability of each cell was observed respectively. *P < 0.05 and **P < 0.01 vs corresponding to normal control (0 mg/L) analyzed by t-test. Data were representative of an experiment that was repeated three times presented as mean ± SD of 12 samples.