Table 1 Oligonucleotide DNAs used for construction of IGFBP-3 promoter mutants.
Plasmid | Cloned fragment* | sense primer** (5' to 3') | antisense primer** (5' to 3') |
|---|---|---|---|
pGL2-270 | -270/+60 | ggacgcgtctggagtgccggggtggc | ggagatctgctgtggaatccaggcag |
pGL2-240 | -240/+60 | ggacgcgtggttcttgtagacgacaa | ggagatctgctgtggaatccaggcag |
pGL2-210 | -210/+60 | ggacgcgtcgggcgtgagcacgagga | ggagatctgctgtggaatccaggcag |
pGL2-180 | -180/+60 | ggacgcgtgcgagtctcgagctgcac | ggagatctgctgtggaatccaggcag |
pGL2-150 | -150/+60 | ggacgcgtggccccggctgctcaggg | ggagatctgctgtggaatccaggcag |
pGL2-120 | -120/+60 | ggacgcgtcccgcagccgtgcctgcg | ggagatctgctgtggaatccaggcag |
pGL2-90 | -90/+60 | ggacgcgtcctcccaacccccactcc | ggagatctgctgtggaatccaggcag |
pGL2-60 | -60/+60 | ggacgcgtccggggcgtgtcctgggc | ggagatctgctgtggaatccaggcag |
pGL2-30 | -30/+60 | ggacgcgttatatacgggccggcgcg | ggagatctgctgtggaatccaggcag |
pGL2-1 | +1/+60 | ggacgcgtagatgcgagcactgcggc | ggagatctgctgtggaatccaggcag |
pGL2-210B*** | -210/+60 | ggacgcgtctgcagcgggcgtgagcacga ggagcaggtgcccgggtgactctcgagct | agctcgagagtcacccgggcacctgctcc tcgtgctcacgcccgctgcagacgcgtcc |