RT-PCR verification of unique gene expression. (A) C4-2 genes: Lane 1–2 ARHE, 3–4 PHF11, 5–6 PLD1, 7–8 PRG-3, and 9–10 ACAS2L. (B) LNCaP genes: Lane 1–2 ETV1, 3–4 EPHA7, 5–6 FLJ12895, 7–8 MBNL2, and 9–10 PCDH11X. Odd lanes are C4-2 and even lanes are LNCaP cDNA. Many genes determined to be unique to a cell line by Affymetrix and MPSS analysis were actually not when assayed by RT-PCR.